A study by the National University of Colombia, first published in the world on this subject, found that an extract Chinese calendar can restore firmness to the cornea. Genipin extract that comes from China Gardenia jasminoides fruit, for several years was patented as a component to alleviate the symptoms of type 2 diabetes. It has also been described as a useful element to prevent after cataract surgery, the capsule is opaque the crystalline lens, allowing focus images on the eye. However, it is the first time is reported as a tool to repair the collagen, the main component of the cornea.
The resistance of the cornea depends on how close they are collagen molecules. When a person suffers a disease of the cornea, such as keratoconus, one of the most common in the world, it is because the cornea loses its elasticity, it retracts back and looks like a balloon. This occurs when the bonding between the collagen molecules and lead to create spaces that it will reduce up to 50% visual quality.
currently to treat keratoconus, there are several treatment modalities such as the implantation of intrastromal rings, some rings that go inside the cornea to prevent twisting. However, this option has its drawbacks, because the ring can be destroyed by the body itself, or it can infect.
recently came another option: the molecular cross-linking, known as crosslinking, a chemical reaction that uses a medicine called riboflavin, which is applied in eye drops and adheres to the cornea using ultraviolet light, highly questionable.
This procedure will create new links between collagen fibers and increases the resistance of the cornea. Thus, improving acuity display in a large percentage of patients
"Despite the encouraging results obtained with this treatment, UV light can damage the endothelium and the corneal epithelium, and in severe cases, compromises the intraocular lens and other tissues," said Marcel Avila, eye cornea and cataract surgeon and author of the project. Just
, Avila takes the crosslinking but, instead of adhering riboflavin (a medication) to the cornea, using ultraviolet light, using the natural compound genipin. How do
The experiment was performed in 25 porcine corneas clean, all aged between 8 and 10 hours post mortem. Specialists cut with a scalpel (surgical knife) two strips of the cornea of \u200b\u200beach animal's eye. A piece of this membrane was preserved as a control (no treatment), and another was treated with genipin, applied topically to 0.10%, 0.25% and 1% in balanced salt solution for 40 minutes.
After treatment, the researchers calculated the elastic behavior of each piece of cornea tested at constant force to the point of rupture. This procedure was done using the Young's modulus, which measures the ease or difficulty to stretch a material. The measurements showed an increase in the strength of the corneas tratadas, dependiendo de la concentración de genipín.
Mientras una córnea control alcanza un estiramiento de 8% con 100 kilopascales (KPa) (el pascal es la unidad de presión del sistema internacional de unidades), para obtener el mismo estiramiento en una córnea tratada con genipín al 0,1% se debe aplicar el doble de fuerza, es decir, 200 KPa, lo que demuestra que se hace más fuerte con el genipín.
Sucesivamente, si se quiere alcanzar ese mismo estiramiento de 8% en una córnea tratada con genipín al 0'25%, la fuerza debe aumentar a 300 KPa, y si es tratada al 1%, la fuerza necesaria para lograr un estiramiento de 8% es de 400 KPa. Así, incrementó la densidad the fibers of the cornea and the decline in the spaces between fiber and fiber became apparent.
The study also led to the discovery that genipin has a positive effect on the resistance of the cornea to bacterial collagenase, a residue left by organisms in a few hours destroys the corneal tissue.
Usually, when an infection occurs, collagenase does degrade 50% of corneal area 20 hours elapsed. In corneas treated with genipin, the total destruction occurs after 220 hours. Dr. Avila consider this a breakthrough because it "gives them a chance to implement treatment and stop the disintegration of the cornea when it becomes infected."
"The use of genipin and was patented for diabetes, that indicates that it is a compound that works for human beings without being toxic. In the field of scientific research that is of great importance, because we have a long road trip as is the testing in humans. Now try the rehabilitation of diseased corneas to ensure that patients get a transplant, "concluded the researcher.
http://www.diariosalud.net/content/view/20274/520/
The resistance of the cornea depends on how close they are collagen molecules. When a person suffers a disease of the cornea, such as keratoconus, one of the most common in the world, it is because the cornea loses its elasticity, it retracts back and looks like a balloon. This occurs when the bonding between the collagen molecules and lead to create spaces that it will reduce up to 50% visual quality.
currently to treat keratoconus, there are several treatment modalities such as the implantation of intrastromal rings, some rings that go inside the cornea to prevent twisting. However, this option has its drawbacks, because the ring can be destroyed by the body itself, or it can infect.
recently came another option: the molecular cross-linking, known as crosslinking, a chemical reaction that uses a medicine called riboflavin, which is applied in eye drops and adheres to the cornea using ultraviolet light, highly questionable.
This procedure will create new links between collagen fibers and increases the resistance of the cornea. Thus, improving acuity display in a large percentage of patients
"Despite the encouraging results obtained with this treatment, UV light can damage the endothelium and the corneal epithelium, and in severe cases, compromises the intraocular lens and other tissues," said Marcel Avila, eye cornea and cataract surgeon and author of the project. Just
, Avila takes the crosslinking but, instead of adhering riboflavin (a medication) to the cornea, using ultraviolet light, using the natural compound genipin. How do
The experiment was performed in 25 porcine corneas clean, all aged between 8 and 10 hours post mortem. Specialists cut with a scalpel (surgical knife) two strips of the cornea of \u200b\u200beach animal's eye. A piece of this membrane was preserved as a control (no treatment), and another was treated with genipin, applied topically to 0.10%, 0.25% and 1% in balanced salt solution for 40 minutes.
After treatment, the researchers calculated the elastic behavior of each piece of cornea tested at constant force to the point of rupture. This procedure was done using the Young's modulus, which measures the ease or difficulty to stretch a material. The measurements showed an increase in the strength of the corneas tratadas, dependiendo de la concentración de genipín.
Mientras una córnea control alcanza un estiramiento de 8% con 100 kilopascales (KPa) (el pascal es la unidad de presión del sistema internacional de unidades), para obtener el mismo estiramiento en una córnea tratada con genipín al 0,1% se debe aplicar el doble de fuerza, es decir, 200 KPa, lo que demuestra que se hace más fuerte con el genipín.
Sucesivamente, si se quiere alcanzar ese mismo estiramiento de 8% en una córnea tratada con genipín al 0'25%, la fuerza debe aumentar a 300 KPa, y si es tratada al 1%, la fuerza necesaria para lograr un estiramiento de 8% es de 400 KPa. Así, incrementó la densidad the fibers of the cornea and the decline in the spaces between fiber and fiber became apparent.
The study also led to the discovery that genipin has a positive effect on the resistance of the cornea to bacterial collagenase, a residue left by organisms in a few hours destroys the corneal tissue.
Usually, when an infection occurs, collagenase does degrade 50% of corneal area 20 hours elapsed. In corneas treated with genipin, the total destruction occurs after 220 hours. Dr. Avila consider this a breakthrough because it "gives them a chance to implement treatment and stop the disintegration of the cornea when it becomes infected."
"The use of genipin and was patented for diabetes, that indicates that it is a compound that works for human beings without being toxic. In the field of scientific research that is of great importance, because we have a long road trip as is the testing in humans. Now try the rehabilitation of diseased corneas to ensure that patients get a transplant, "concluded the researcher.
http://www.diariosalud.net/content/view/20274/520/
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